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TJUHBB

Blood Collection

Standard Operating Procedure (SOP 05.001.2)

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PURPOSE

Blood samples are drawn from patients that have been through the informed consent process and agreed to participate in the tumor biobank program. Blood samples are obtained by personnel qualified to draw blood from patients in the cancer center, the hospital or in the physician’s office. The purpose of this document is to outline standardized procedures for the TJUHBB biobank to follow for blood collection.

SCOPE

This standard operating procedure (SOP) describes how blood should be drawn. This SOP does not cover detailed safety procedures for handling blood, and personnel must follow institutional bio-safety guidelines. When blood collection is subcontracted, the subcontractor must follow institutional guidelines.

REFERENCE TO OTHER TJUHBB SOPS OR POLICIES

  • TJUHBB POL 02.001 Chemical Hygiene Plan
  • TJUHBB POL 02.002 Laboratory Safety Policy
  • TJUHBB POL 01.001 Quality Management Plan
  • TJUHBB SOP 08.02.002 Blood Processing and Storage

ROLES AND RESPONSIBILITIES

This SOP applies to all qualified tumor biobank personnel and clinical staff at the collection centers that are involved in recruiting patients and the acquisition of informed and voluntary consent. This may include the following personnel:

Tumor Biobank PersonnelResponsibility
Tumor Bank ManagerObtain patient consent, transport blood, process blood
Anesthesiology DepartmentDraw blood from patient

MATERIALS, EQUIPMENT AND FORMS

The materials, equipment and forms listed in the following list are recommendations only and may be substituted by alternative/equivalent products more suitable for the site-specific task or procedure.

Materials & EquipmentMaterials & Equipment (Site Specific)
Evacuated blood collection tubes for plasma (e.g. Lavender top tube with EDTA)2 x 4 mL Lavender top tubes
Needles of appropriate gauge number1
Tourniquet1
Alcohol wipes (70%) isopropyl alcohol2
Gauze sponges for application to site from which needle has been withdrawn4
Adhesive bandages/tape to protect venipuncture site after collection1
Needle/sharps disposal unit1
Gloves (non-latex recommended) worn to protect patient and phlebotomist1 pair
Syringes that may be used in place of evacuated collection tubes in certain circumstances.2 x 10 mL

DEFINITIONS

See the TJUHBB Glossary.

PROCEDURES

This procedure is intended to ensure that blood samples will be obtained from consented participants in a safe and efficient manner while eliminating the risks of contamination. Blood products are a precious resource in the tumor biobank and procedures must be followed to obtain products with high integrity and quality.

Timing for Blood Collection

  • Preferably, blood collection should be done pre-operation and as close as possible to the time when the tissue is donated to the biobank or at an alternative time, if appropriate for the research study.

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Peri-operative Blood Draw

If blood collection is not possible pre-operatively (either due to repeated unsuccessful venipuncture or patient refusal) peri-operative blood is an acceptable substitute. The blood is drawn after anesthetic induction is achieved, either through venipuncture or withdrawal from an existing arterial line.

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Documentation

If peri-operative blood is taken, it must be noted in the biorepository database that the blood draw was peri-operative and specify the method by which the blood was drawn (venipuncture vs arterial-line).

  • Identify the person responsible for processing the blood collection and arrange for timely processing (Refer to TJUHBB SOP 08.02.002 – Blood Processing and Storage for further details).

Blood Collection Procedure - Preparation

  • Personnel qualified by training to draw blood must perform blood collection.
  • Identify the patient, verify identification, and check that informed consent has been obtained.
  • Record the time that blood was drawn.
  • Assess patient’s physical and mental disposition and determine if this is the appropriate time to draw blood.
  • Be courteous, professional, and sensitive to the patient’s needs. Ensure that all communications are discreet and respectful of patient confidentiality.
  • The anesthesiologist will draw blood for the biobank.

Transport of Blood Sample to Pathology or Biobank Lab for Processing

  • Verify patient information (in keeping with privacy and ethical policies) and ensure that it corresponds with the information on the labels on blood collection tubes.
  • The biobank manager should transport labelled tubes to the specified area at the biobank for processing blood samples.
  • Transport EDTA tubes at room temperature unless otherwise specified.

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Warning

Do not allow the samples to freeze or be exposed to an ambient temperature of greater than 25° C.

APPLICABLE REFERENCES, REGULATIONS AND GUIDELINES

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